Effects of Cell Density, Aging and Media Formulation on CHO Antibody Expression: Feasibility of Titers >1 g/L

Flow electroporation provides a means of fully scalable, highly efficient transient gene expression that results in antibody titers >1g/L and production of multiple grams of antibody from non-engineered CHO-S cells. In this poster, data using flow electroporation via the MaxCyteSTX® are presented that examine the effects of pre-transfection parameters such as media supplementation, cell density, and cell passage number on CHO-based antibody production.