Efficient DNA Extractions for the CRISPR Toolkit

CRISPR genotyping is a necessary step in the ever-growing area of CRISPR-Cas9 genome modification to identify which cell-lines have the desired modification. This process can often be laborious and, with the inherent loss of DNA from traditional DNA extraction methods, long proliferation times are required to ensure an adequate number of cells and therefore adequate quantities of DNA. The MicroGEM rapid protocol, with its purely enzymatic approach using a thermostable proteinase and buffer system, aims to allow researchers to shorten proliferation time, limit extraction time and process a large number of samples simultaneously to streamline the CRISPR genotyping workflow to a few, simple, efficient steps.