Efficient Plasmid DNA Isolation from Bacterial Culture with Thermo Scientific KingFisher Flex and KingFisher Pure Plasmid Kit

Efficient plasmid DNA purification from bacterial cultures is key to many molecular-based experiments. This application note describes an automated process for plasmid DNA purification from bacterial cultures. The method integrates the use of the Thermo Scientific™ KingFisher™ Pure Plasmid Kit and the KingFisher Flex platform for efficient purification of plasmids of various sizes. The system was also used to purify plasmids containing recombinant antibody sequences for sequence identification.