Enhance Your Calcium Screens With FLIPR Calcium 6 Assay Kits

In this application note, the use of FLIPR Calcium Assay Kits from Molecular Devices is demonstrated in the fluorescent screening of G-protein coupled receptors (GPCRs), ion channels, and other calcium sensitive targets. Using a novel dye formulation to further enhance the calcium flux assay signal window, assay robustness was increased, while providing greater assay protocol flexibility. Certain cells such as Chinese Hamster Ovary (CHO) cell lines have an anion-exchange protein that requires the use of an anion reuptake inhibitor, such as probenecid, to retain commonly used calcium indicators within the cytosol.