Enhanced LC-MS/MS separation of Δ⁸ and Δ⁹-THC metabolites and other cannabinoids extracted from urine samples using a polymeric SPE column

In this application note, UCT, Inc. presents a method addressing the challenge posed by the increased use of Δ⁸-THC products. Drug testing labs face the need to separate parent THC isomers Δ⁸ and Δ⁹-THC. THC metabolites, particularly isomeric ones like Δ⁸-OH-THC and Δ⁹-OH-THC, present a challenge. Additionally, new cannabinoids such as Δ¹⁰ and Δ^6a,10a-THC, and THC-O-Acetate are emerging. The note introduces an LC-MS/MS method employing UCT’s SelectraCore^® PFPP column to separate 16 cannabinoids, including isomeric metabolites of Δ⁸ and Δ⁹ -THC. Solid-phase extraction from urine is detailed using UCT’s Styre Screen^® HLB column.