Evaluating Unfolding Reversibility using Temperature Cycle Measurements with the Prometheus and PR.TimeControl Software

The conformational stability of proteins is of central importance for a large number of commercial products, ranging from biopharmaceutics to industrial enzymes. This application note demonstrates the capabilities of the new PR.TimeControl software, which enables easy evaluation of unfolding reversibility for instruments of the Prometheus series. This can be done either by repeat cycles between two defined temperatures (“temperature cycling”), or by a repeated increase in the maximum temperature for each cycle (“incremental cycling”).