Evaluation of a New Intracellular Calcium Fluorescent Reagent Using the FDSS6000

In this application note, the fluorescence spectral properties of DiscoveRx Calcium assay reagent are evaluated, and are compared with the parameters of the FDSS6000 calcium assay filter set. Data obtained in several experimental conditions are presented, and allow the assessment of DiscoveRx Calcium Assay reagent and Hamamatsu FDSS6000 reader as an appropriate environment for running GPCR calcium release functional HTS.