Evaluation of Enzymatic DNA Digestion as an Alternative to Mechanical DNA Fragmentation (Sonication) for Targeted NGS using the SureSeq™ Myeloid Panel

This application note presents enzymatic digestion using Oxford Gene Technology’s NEBNext^® dsDNA Fragmentase^® as an alternative to mechanical fragmentation. DNA fragmentation is a crucial first step in the preparation of libraries for next generation sequencing (NGS). Mechanical shearing (sonication) is currently the gold-standard for fragmentation but it requires a significant upfront capital investment. Oxford Gene Technology (OGT) has evaluated an alternative method of fragmentation using the NEBNext^® dsDNA Fragmentase^®.