Evaluation of Membrane Potential Assay Kit Using the FDSS/µCELL Imaging Plate Reader: Detection Ion Channel in Nicotine Acetylcholine Receptors (nAChRs)

Membrane Potential Assay Kits detect ion channel modulation by increasing or decreasing the fluorescent signal as cellular membrane potential changes. The fluorescent signal increases in intensity during membrane depolarization as dye follows the positively charged ions inside the cell. During membrane hyperpolarization, fluorescent signal decreases in intensity as dye follows the positively charged ions out of the cell. Membrane Potential Assay Kits are uniquely suited for use with the simultaneous pipette and read capability of FDSS/µCELL to capture fast kinetics associated with ion channel activation.