Exosome characterization with FFF-MALS-DLS

In this whitepaper, Waters | Wyatt Technology emphasize the growing significance of studying exosomes and extracellular vesicles (EV) in molecular biology and medical research. Biophysical characterization and isolation play a central role in these studies, forming the basis for further analyses. The paper introduces field-flow fractionation coupled to online multi-angle and dynamic light scattering (FFF-MALS-DLS) as a versatile biophysical technique, providing detailed characterization of exosome physical properties such as size and charge, and facilitating size-based isolation.