Expansion and Differentiation of H7 Stem Cells in Chemically Defined Medium Using Corning® Synthemax™-T Surface

This application note describes the use of Corning® Synthemax™-T Surface to support long-term self-renewal and differentiation of H7 hESCs in chemically defined medium. H7 hESCs were successfully cultured for >10 serial passages. Cells also demonstrated a stable doubling time of 41 ± 5 hours and a high level of expression of hESC phenotypic markers.