Extraction of 2’-MOE phosphorothioate from tissue using Clarity OTX SPE

In this application note, Phenomenex discusses the rising importance of oligonucleotides as a therapeutic option with broad applications. Analyzing oligos in bioanalytical workflows poses unique challenges due to their distinct properties. Achieving relevant limits of quantitation and linearity in pharmacokinetic assays is especially tough due to their low dosing. To overcome these challenges, the note explores a novel single-step solid-phase extraction (SPE) method called Clarity® OTX for extracting a specific splice-switching oligo (SSO) from central nervous system (CNS) tissue.