Extraction of Malachite Green, Crystal Violet and their Leuco Metabolites from Salmon Using QuEChERS and EVOLUTE CX for LC-MS-MS Analysis

This application note describes the extraction of malachite green, leuco malachite green, crystal violet (gentian violet), leuco crystal violet (leuco gentian violet) and brilliant green from oily fish tissue using enhanced dispersive SPE followed by EVOLUTE CX clean up. The work focuses on ease of use when compared to other methods, and describes a QuEChERS based approach incorporating the use of aluminium oxide in enhanced dispersive SPE to reduce the levels of fat in the final extract.