Factor Based Quantification of Nucleic Acid Concentrations in the Eppendorf PlateReader AF2200

This Application Note will demonstrate how concentration determination via the sample-specific extinction factor is possible with the Eppendorf PlateReader AF2200 in combination with the Eppendorf Microplate UV-VIS. This approach eliminates the need for a standard curve, thus saving both time and money. The results obtained are compared to reference measurements in a standard cuvette as well as to the established method of using a standard curve.