Fast, Accurate and Reproducible Method for Simultaneous Quantitation of Pyridoxal 5’-phosphate and Pyridoxic Acid in Human Plasma by HPLC with Fluorescent Detection

Fluorescence detection has been used nearly exclusively by a majority of reference laboratories in HPLC analysis of vitamin B6 due to its selectivity and high sensitivity. However, the published derivatization procedures suffer from tedious sample preparation and short HPLC column lifetimes. This study aimed to simplify the derivatization and sample preparation steps when analyzing PLP and pyridoxic acid (PA) by HPLC-FLD.