Fast Molecular Interaction Screening of Epigenetic Gene Regulator G9a with Fragments from a Large Chemical Space

Dianthus swiftly detects and quantifies molecular interactions in solution, independent of the molecular mass of the two interacting molecules, making it a perfect match for fragment-based screening with compounds representing a large chemical space. In this application note, data from single-dose screening of a fragment library is shown with a broad molecular mass and complexity range against the target molecule histone methyltransferase (HMT) G9a, followed by affinity constant determination (Kd) for a selection of hit molecules.