Fast Preparative Column Liquid Chromatography

The technique presented in this application note, FAST PCLC (preparative column liquid chromatography), reduces analysis time and increases throughput. For example, a 5-μm column can handle a throughput of 1 gram in about one-sixth of the time required for a 30-μm column. Complete preparative separations can be developed on the same system with the same column. Having completed method development, the user can leave the system to operate unattended. Repetitive cycles, rather than scale-up, substantially reduce labor and column costs. The instrument can then be used to check for purity of collected fractions.