Fast Separation of Triacylglycerols in Oils using UltraPerformance Convergence Chromatography (UPC2)

UltraPerformance Convergence Chromatography™ (UPC2®) is a novel technology that applies the performance advantages of UPLC to supercritical fluid chromatography (SFC). Combining the use of supercritical CO2 with sub-2-μm particle columns, UPC2 represents an analysis technique that is orthogonal to reversed-phase LC and can be used to solve many troublesome separations that challenge conventional LC or GC analyses. It also generates less solvent waste as compared to liquid chromatography. This application note presents studies using UPC2 coupled with ultraviolet (UV) detection, mass spectrometry (MS), and evaporative light scattering (ELS) detection for the separation of triacylglycerols in tobacco, corn, sesame, and soybean seed oils.