Feeder-Free Expansion of Human Induced Pluripotent Stem Cells (hiPSC) on Corning® Synthemax® II-SC Substrate Coated Surface in Defined Medium

This report describes the use of Corning Synthemax II-SC Substrate, coated on Corning CellBIND® Surface 6 well plates, for the multi-passage expansion of human iPSC in defined medium. Cells maintained consistent doubling time, expression of pluripotency markers, and normal karyotype when cultured on Corning Synthemax II-SC Substrate for seven sequential passages in mTeSR®1 medium.