Fibroblast-derived VEGF Regulates Endothelial Cell Growth and Differentiation in a Co-culture Angiogenesis Model

Using a compact fluorescent imaging instrument with a kinetic co-culture model of angiogenesis, involving GFP-infected HUVECs and normal human fibroblasts, this scientific poster demonstrates the ability to visualize and quantify all stages of in vitro angiogenesis in a time-dependent manner for up to 14 days. Algorithms were developed to easily quantify tube length, area, and branching to measure the effects of pro-angiogenic and anti-angiogenic agents. In addition, the contribution of endogenous VEGF on tube formation using anti-VEGF antibodies was also examined.