Flow Electroporation for Vaccine Development and Production: From Subunit Vaccines to Ex Vivo Immunotherapy

MaxCyte flow electroporation is a universal, clinically validated transient transfection platform for rapid, high-quality cell transfection. This white paper reviews MaxCyte flow electroporation and its applications in the development and production of vaccines and cellular immunotherapies. Flow electroporation enables (co)transfection of a wide range of cells with DNA, RNA, proteins, or cell lysates using single-use processing assemblies for cGMP, “plug-and-play” manufacturing of recombinant proteins and vaccines. This technology combines superior performance, broad applicability, and ease of use to create a fully scalable, highly reproducible, and regulatory-compliant transfection method.