FLOWSCRIPT™ Platform Detects Genotypic and Phenotypic Markers Associated with HPV Infection and Cancer Progression

This poster demonstrates the use of an assay that employs a novel in situ hybridization technique utilizing cocktails of oligonucleotide probes specific to multiple targets within the E6 and E7 genes to detect transcripts from most known variants of high risk HPV. Fixed and permeabilized cells are hybridized with probes and analyzed by flow cytometry for E6/E7 transcript expression. Each probe contains a fluorescent label and a quenching molecule whereby no signal is observed in the absence of target. During hybridization, the probe anneals to the target sequence, thereby emitting a detectable signal.