Fluorescence-Based Analysis of Drug Sensitivity in Tumor Microtissues Using the Infinite® 200 PRO Multimode Microplate Reader

To gain full benefit of more organotypic tumor culture systems, appropriate assays which enable high-throughput analysis over time without disrupting the tissues have to be adopted. This study shows the development of microtissue models harboring fluorescence reporter for non-disruptive drug sensitivity testing of homotypic and heterotypic colon cancer spheroids. Detection of the GFP signal was performed using the Infinite 200 PRO multimode microplate reader.