Fluorescence Quenching in Cell-Based Assays Can Be Dye Specific: A Case Study Using Both Fura-2 AM and Fluo-4 AM Calcium Reporter Dyes Using the FDSS6000

In this case study we screened a panel of 3500 compounds for antagonism using both Fura-2 AM and Fluo-4 AM dye loaded cells. Results reveal some compounds mediate strong inhibition, reported as no change in fluorescence upon agonist addition, against one dye-loaded cell model and not the other, suggesting dye-specific quenching.