Fluorescent Immunodetection Using the SNAP i.d.™ Protein Detection System

Recent advances in fluorescent dye chemistry and blotting membranes coupled with improvements in instrumentation have accelerated the application of fluorescence detection methodologies to western blotting. The SNAP i.d. Protein Detection System has been developed to substantially shorten the time required for fluorescent immunodetection through the use of vacuum. This application noted discusses how to the SNAP i.d. Protein Detection System can be optimized for this purpose.