Fluorescent western blotting – A guide to multiplexing

Explore fluorescent western blotting and its advantages, including quantitative accuracy, stable signals, and the ability to evaluate multiple protein targets simultaneously through multiplexing. Techniques like 2-plex and 4-plex experiments enhance efficiency and allow the analysis of complex pathways. Discover how with the appropriate experimental setup, the Thermo Scientific™ Invitrogen™ iBright™ FL1500 Imaging System can perform up to a 4-plex fluorescent western blot.