Functional characterization of 3D neurospheres assembled using iPSC-derived neurons and astrocytes

In this application note, Molecular Devices describes methods for assembling normal human iPSC-derived cell types, including glutamatergic neurons, GABAergic neurons, and astrocytes into 3D neurospheres for compound profiling. Neural organoids and 3D spheroids are a rapidly developing technology with great potential for understanding brain development and neuronal diseases. They provide a more advanced and biologically relevant system for basic research and high-throughput drug discovery, including compound profiling and toxicity testing.