Fundamentals of Embryo Development Uncovered in Real Time using High-Speed Light Sheet Microscopy

In this study, researchers employed the light sheet microscopy approach of SPIM and the fast acquisition speed of the sCMOS camera to study the endoderm of the Zebrafish during early stages of development. Early endoderm migration is of immense importance for organ formation, as defects in cell movement during gastrulation result in the duplication or mis-positioning of organs. High-speed light sheet microscopy was utilized in conjunction with Zyla 5.5 and Zyla 4.2 sCMOS cameras from Andor Technology; ideally suited detectors for high-speed light sheet microscopy.