Guidelines for protein purification at low temperature

11 Oct 2023

Protein purification at low temperatures is crucial for preserving protein integrity and preventing proteolytic degradation, as well as managing protein aggregation tendencies. This application note offers general principles for successful protein purification in cold environments, with ÄKTA™ systems, chromatography resins, and prepacked columns typically performing well at 4°C to 8°C, similar to room temperature results.

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