High-Content 3D Toxicity Assay Using iPSC-Derived Hepatocyte Spheroids

There is increasing interest in exploring the use of three-dimensional (3D) spheroids for modeling developmental and tissue biology with the goal of accelerating translational research in these areas. As a result, the development of higher throughput quantitative assays using 3D cultures is an active area of investigation. In this study, we developed and optimized methods for the formation of 3D liver spheroids derived from human iPS cells as well as confocal imaging and analysis methods for toxicity assessment.