High-Content Assay for Morphological Characterization of 3D Neuronal Networks in A Microfluidic Platform

The focus of this application note is to develop a high-throughput 3D neurite outgrowth assay using iPSC-derived neurons developed in the microfluidic OrganoPlate^® platform, with the goal of establishing 3D models for neurodegenerative diseases and neurotoxicology screens. The OrganoPlate^® is a high-throughput platform that combines the most recent advances in 3D cell culture, Phaseguides™ and microfluidics. The OrganoPlate contains 96 tissue chips suitable for long-term culture of live cells, is amenable for screening purposes, and is compatible with standard laboratory equipment or automated systems, such as the ImageXpress^® Micro Confocal High-Content Imaging System.