High-plex multiomic analysis in FFPE tissue at single-cellular and subcellular resolution by spatial molecular imaging

The Spatial Molecular Imager (SMI) is an automated microscope imaging system with microfluidic reagent cycling, for high-plex, spatial in situ detection of multiomic targets (RNA and protein) on FFPE and other intact samples with subcellular resolution. Here, the chemistry and applications of an SMI are described to address many of the current unmet needs in spatial high-plex profiling of RNA and protein expression.