High-Resolution Analysis of Tryptic Digests of Bovine Serum Albumin using UHPLC with Photodiode Array Detection

Peptide mapping is a standard testing method for biomedicines. This method requires HPLC separation of peptide segments digested using enzymes or chemicals. In this application note, U.S. Pharmacopoeia (USP), the European Pharmacopoeia (EP) and the Japanese Pharmacopoeia (JP) protocol is followed to analyze bovine serum albumin using UHPLC with photodiode array detection.