High-Resolution Cation-Exchange Alternative to Peptide Mapping for Protein ID and QA/QC

Although a reversed-phase separation is the typical choice for separating peptides, high-resolution ion-exchange chromatography is an alternate method that provides additional information and a different selectivity. The work shown here demonstrates that strong cation-exchange chromatography can provide good resolution for peptide mapping, and thereby serve as an alternate or supplementary method for separating peptides.