High Speed Analysis of Dabsyl Amino Acids in Collagen using UHPLC

Collagen, one of the proteins that has been recently attracting attention from the viewpoint of health and beauty. In order to measure the constituent amino acids of proteins or peptides such as collagen, sample preparation such as hydrolysis is required. A DAB-Label kit is available from JASCO for amino acid derivatization using Dabsyl chloride as reagent. In this report, collagen was hydrolyzed with the DAB-Label kit and the derivatized amino acids were separated by Ultra High-performance Liquid Chromatography (UHPLC) using UV detection.