High-Speed MALDI-TOF/TOF Imaging of Mouse Brain Tissue Performed on Intact Proteins and After On-tissue Digestion

In this application note, the capability and flexibility of the MALDI-TOF/TOF system for imaging of intact proteins and tryptic peptides (after tissue digestion) is demonstrated. The on-tissue digestion approach benefits from significantly higher mass resolution and accuracy that can be achieved for tryptic peptides in reflector operation mode, as well as from aditional specificity provided by MS/MS analysis.