High-Throughput Assays for Characterizing the Viability and Morphology of 3D Cancer Spheroid Cultures

The goal of this study was to optimize cell culture and develop new imaging and analysis methods that can be used for compound screening through assessment of multiple phenotypes in human cancer 3D models. This poster demonstrates concentration-response effects of selected test compounds on HTC116 cell spheroids and illustrates how the proposed methods may be used for high-content high-throughput compound screening and evaluation of anti-cancer drugs.