High-Throughput Gene Expression Analysis of 3D Airway Organoids

In this application note, a novel high-throughput gene expression analysis technology — the nanoString nCounter^® platform — is used to characterize gene expression of airway organoids generated from healthy, and asthmatic, primary bronchial cells. The nanoString nCounter PlexSet assay provides a simple and cost-effective solution for multiplex sample analysis of up to 96 custom probes across 96 samples per run. The assay uses whole cell lysate, without the need for RNA purification, and was simple to incorporate into the airway organoid workflow. The end result is high-throughput gene expression data without amplification, cDNAconversion or library prep.