High Throughput Large Scale Genotyping in Soybean Utilizing Novel Microfluidic Reagent Dispensing Technology

Recent advances in DNA marker/QTL (gene) discovery and marker detection technology have great impacts on crop improvement through marker-assisted selection. To discover and deploy the DNA markers in plant breeding programs, establishing low cost, high throughput, large scale and reliable and flexible marker genotyping platform is one of key factors. Implementing high-throughput workflows for SNP genotyping platforms such as TaqMan and KASP assays requires accurate dispensing of assay master mixes and reagents. This poster demonstrates the use of the Mantis instrument within the marker/QTL discovery and molecular breeding processes in a soybean program.