High-throughput Miniaturization of Cytochrome P450 Time-dependent Inhibition Screening Using the Echo® 525 Liquid Handler

Early assessment of the ADMET (Absorption, Distribution, Metabolism, Excretion and Toxicity) properties of drug candidates has become a critical part of modern drug discovery. Results from time-dependent CYP inhibition experiments can predict clinically relevant drug-drug interaction potential. Conventionally the assay is run with large reaction volumes to reduce DMSO concentration. In this application note it is demonstrated that miniaturized and simplified time-dependent CYP inhibition assays can be efficiently conducted with the Echo 525 liquid handler.