High-Throughput Single Cell Gene Expression Profiling by Multiplex qPCR

Single cell gene expression analysis is a powerful technique that provides a unique and insightful perspective on biological pathways and processes. This scientific poster presents a robust workflow that was used to study how concentration of retinoic acid affects differentiation of NTera2 cells, a human embryonic stem cell model system. The workflow enables fast and accurate analysis of up to 100 genes in isolated single cells and has the potential to enable researchers to investigate cell heterogeneity in a cost-effective way.