High-Throughput SISCAPA-Based Peptide Quantitation Using an Agilent RapidFire High-Throughput Mass Spectrometry System

Mass spectrometry (MS) provides an effective method for the reproducible quantitation of biomarkers. The limited sensitivity of such assays and the low throughput associated with peptide chromatography represent major limitations in the validation of candidate biomarkers. This application note describes a high-throughput SISCAPA workflow that has been developed using RapidFire/MS technology to enable sensitive quantitation of peptides from plasma digests with a sample cycle time of approximately 10 seconds.