High-throughput, three-dimensional assay development

3D models, such as the use of spheroids, are being increasingly utilized for drug discovery. To meet the demands of high-volume drug discovery programs, a high-throughput system is essential. In this application note, INTEGRA Biosciences demonstrates robust quality data for assay development and high-throughput screens generated via an automated method for seeding, dosing, and assaying spheroids. This method uses Corning 1536-well spheroid microplates with the assistance of INTEGRA Biosciences’ liquid handling instruments: the VIAFILL and the VIAFLO 384.