Higher multiplexing with NeuCode SILAC metabolic labelling

This application note describes a method for multiplex quantitation of stable isotope labelling using amino acids in cell culture (SILAC) using neutron encoded amino acids (NeuCode™). Samples were combined for multiplex analysis using a Thermo Scientific™ Orbitrap™ Fusion or Orbitrap Elite mass spectrometer and MaxQuant software modified for NeuCode data analysis was used for peptide identification and quantification. This application note highlights the capability of NeuCode to resolve multiple isotoplogs of the same nominal mass at high resolution.