Highly-efficient miRNA Isolation using the Agencourt FormaPure and RNAdvance Cell v2 Kits and Biomek Automated Extraction Methods

This application note describes an efficient and robust miRNA extraction method using Agencourt SPRI (Solid Phase Reverse Immobilization) technology and Biomek automation. The SPRI procedure is an easy, rapid, high yield, and automation-friendly nucleic acid purification method that does not require organic solvents, centrifugation or filtration steps. This method uses carboxyl-coated magnetic particles that reversibly bind nucleic acids in the presence of binding buffers and crowding reagents.