ResourceSeparations
Highly polar antibiotic separation
25 Mar 2022In this application note, an LC-MS separation of the highly polar antibiotics amikacin and tobramycin is presented using the Cogent Diamond Hydride Column. The two peaks are baseline separated chromatographically, but further specificity is also obtained by the use of extracted ion chromatograms (EICs) for the [M+H]+ ion of each compound.