HLA typing using long-range PCR and next-generation sequencing

Human leukocyte antigen (HLA) typing is crucial for eligible transplants between recipient and donor, where HLA settings have to match to minimize the risk of rejection from an immune response. Several methods can be applied for HLA typing such as endpoint PCR, qPCR, Sanger sequencing, and next-generation sequencing (NGS).

Explore a HLA typing workflow using the GenDx NGSgo Library Full Kit and NGSgo-MX11-3 amplification kit. The initial long-range PCR and all library preparation steps were performed using the Biometra TAdvanced 96 SG that ensures high temperature accuracy and uniformity, minimizing the risk of PCR bias and evaporation for optimal PCR specificity and product yield.