Host Cell Protein Removal with Sartobind STIC® PA Pico

This application note details a method for host cell protein removal using the Sartobind STIC^® PA pico from Sartorius. Sartobind^® STIC (Salt Tolerant Interaction Chromatography), is a membrane concept based on weak anion exchange chemistry that was developed to facilitate a wider design space for FT-AIEX membrane chromatography at commercial scale. Sartobind STIC PA is able to overcome the limitations of HCP removal at high conductivities, allowing for >10 times reduced sample consumption and substantial cost savings for virus validation studies.