How Does the Analysis of Biomolecules Benefit from UHPLC?

Ultra high performance liquid chromatography (UHPLC) provides improved separation speed, throughput, and sensitivity by employing stationary phase particles of around 2 μm or smaller. Biomolecules have, apart from their size, other differences from small molecules (e.g. charges, complex structures) that make the application of UHPLC less straightforward. This article discusses the rationale of using UHPLC in life sciences. It also discusses the requirements for a true bio UHPLC system and shows examples of relevant biomolecule separations.