How to Avoid Formation of Oils and Gums When Drying HPLC Fractions

Traditional concentration of reverse phase HPLC fractions to dryness in a Genevac HT evaporator is a fast and safe process. However, for certain compounds a fully dried result can be hard to achieve due to compound interactions with water boosting up the boiling point making drying difficult. Some compounds also tend to naturally form a gum or oil. In such cases, subsequent characterization by NMR normally indicates the presence of residual solvent, whilst samples which are not completely dry are of little use in biological assays since accurate weights cannot be obtained.This application note presents the Lyospeed™ method for avoiding these problems.